To prepare Shaoyao Gancao Dripping Pills (SGDP) and determine its main contents of paeoniflorin，liquiritin and glycyrrhizic acid by HPLC Method，and to provide important reference and parameters for the effective quality control of SGDP.Methods:SGDP was prepared by water decoction，ethanol precipitation and purification of HP-20 macroporous resin.The main contents paeoniflorin，liquiritin and glycyrrhizic acid of SGDP were determined by HPLC-DAD methods at 230 nm，276 nm and 250 nm，respectively.The appearance，weight difference and solution time were determined according to Chinese Pharmacopoeia (2015).Results:SGDP was successfully prepared and the main contents of paeoniflorin，liquiritin and glycyrrhizic acid ammonium salt of SGDP were (32.66±0.46) mg/g，(9.23±0.23) mg/g，and (17.48±0.86) mg/g，respectively，and could be detected simultaneously by HPLC method with mean recovery rate of 111.23%，96.04% and 104.43%，respectively.The indexes of appearance，weight difference and solution time were consistent with the standards of the pharmacopoeia.Conclusion:The preparation techniques of SGDP was rational and practicable.HPLC method was sensitive，accurate，and reproducible.It could be used to determine paeoniflorin，liquiritin and glycyrrhizic acid of SGDP simultaneously and provide important reference for the quality control of SGDP.