To explore the effects of dendrobium huoshanense on serum IL-2，IL-6 and antioxidation in mice with kidney-yin deficiency. Methods:A total of 96 healthy Kunming male mice were divided into normol group，model group，Dendrobium candidum group (3 g/kg)，Dendrobium huoshanense (0.33 g/kg，1 g/kg，2 g/kg，and 3 g/kg) four groups with the correspondent drugs for 14 days. In addition to normal group，the mice in each group were given thyroxine solution 160 mg/kg every afternoon to establish the kidney-yin deficiency model. The changes of hair，food intake，autonomous activity in mice were observed，and the body weight，thymus index and spleen index were measured，and the level of IL-6 and IL-2 in serum，the content of SOD，GSH-Px，CAT and MDA in liver tissues were detected separately. Results:Dendrobium huoshanense can obviously alleviate the yin deficiency symptoms of mice，such as yellowing dull hair，dry stool，yellow urine，restlessness，irritability and increase the body weight (P<0.01，P<0.05). Dendrobium huoshanense could obviously relieve the deficiency of the mice，and increase the body weight (P<0.01，P<0.05). The 1 g/kg，2 g/kg and 3 g/kg Dendrobium huoshanense groups could improve the thymus index and spleen index (P<0.01)，and reduce the serum IL-6 (P<0.05，P<0.01)，increase the level of SOD，GSH-Px and CAT in liver tissue of yin deficiency mice (P<0.01)，and reduce the level of MDA in liver tissue (P<0.05，P<0.01); and the effects of increasing SOD，CAT and the MDA decreasing rate of these 3 groups are faster than that of Dendrobium candidum groups. Conclusion:Dendrobium huoshanense can enhance the immune regulation and the ability of anti-oxidation of the body with kidney-yin deficiency，and the former effect is similar to that of Dendrobium candidum and the later effect is better than that of Dendrobium candidum.