To observe the effects of Chinese Medicine BZL recipe(composed of Atractylodes macrocephala polysaccharide，chlorogenic acid，and geniposide)on non-alcoholic steatohepatitis(NASH)and intestinal mucosal injury induced by high-fat diet in mice. Methods:Twenty seven male C57BL/6J mice were randomly divided into the normal group，the model group，and BZL group，with 9 rats in each group. Except the normal group，the other groups were induced by NASH. From the 14th week，mice in BZL group were administrated with BZL recipe by gavage daily，and the other mice were administrated with same dose distilled water. Till the end of 17th week，the mice were killed for collection materials. The body weight，food intake，liver-to-body ratio，liver histopathology(HE and Sirius red staining，SAF score)were observed in each group. Plasma ALT，GGT activity，LPS level，liver tissue TG content，liver tissue collagen type I and type IV，endotoxin receptor CD14，TLR1，TLR2，TLR4，TLR9 and inflammatory factors IL-1β，TNF-α mRNA expression(real-time PCR)，intestinal histopathology(HE staining)，large intestine tissue tight junction zo-1，occludin，claudin mRNA expression(real-time PCR)and protein expression(immunofluorescence staining)were detected.Results:In the high-fat diet induced NASH model，plasma ALT，GGT，LPS and liver tissue TG levels in the model group were significantly higher than those in the normal group(P<0.01); liver tissue showed fat deposition，inflammation，balloon-like changes and fibrosis. SAF score was significantly higher than the normal group. Liver tissue collagen ColI，ColIV mRNA increased significantly，and CD14，TLR1，TLR2，TLR4，TLR9 and IL-1β，TNF-α mRNA expression increased(P<0.05，P<0.01); The pathological changes of intestinal tissue were not obvious under light microscope. The expression of zo-1，occludin and claudin mRNA in the large intestine was decreased(P<0.01)，and the immunofluorescence staining of zo-1 and occludin was decreased. BZL recipe can reduce plasma ALT，GGT，LPS，liver tissue TG content(P<0.05，P<0.01)，improve SAF score，reduce liver tissue collagen ColI，ColIV mRNA expression(P<0.01)，reduce CD14，TLR2，TLR4，TLR9，IL-1β and TNF-α mRNA expression(P<0.05，P<0.01)，restored the expression of zo-1 and occludin mRNA in the large intestine，and restored the immunofluorescence staining of zo-1 and occludin. Conclusion:BZL recipe can effectively alleviate NASH induced by high-fat diet，which is closely related to the protection of intestinal mucosa，inhibition of LPS intestinal leakage and TLRs and inflammatory factors in liver tissue.