To explore the therapeutic effects of Pearl Scald Ointment in rats with ulcerative colitis(UC)induced by dextran sulfate sodium(DSS). Methods:The UC rats induced by DSS were randomly divided into the normal control(NC)group，model group，Mesalazine(Mes)group，low and high dose groups of Pearl Scald Ointment(Pso)with 10 rats per group. After administration for 10 days，the changes of body weight，colon length，pathological scores and inflammatory factors in rats were observed and analyzed. Results:Compared with the model group，the disease activity indexes，colon lesion scores，HE staining lesion scores and levels of inflammatory factors of rats in each dose group of Pso were significantly decreased. The body weight and shorted colon length caused by UC were improved with statistically significant differences(P<0.05). Conclusion:Pso has significant improvement effects on rats with UC induced by DSS，which suggests that it has a certain therapeutic effects on rats with UC.