To study the effects of Bushen Huoxue recipe on knee osteoarthritis(KOA)patients with kidney deficiency and blood stasis and its influence on serum cytokines. Methods:A total of 262 KOA patients with kidney deficiency and blood stasis who were treated in the first people's Hospital of Jingzhou from February 2016 to November 2017 were selected. These patients were divided into the control group(n=131)and the observation group(n=131)according to random number table method. The control group was treated with glucosamine sulfate capsule and celecoxib capsule，while the observation group was treated with Bushen Huoxue recipe and the 2 groups were treated for 8 weeks. The clinical effects and adverse reactions of the 2 groups were observed. The total score of TCM symptoms，visual analogue score(VAS)，WOMAC total score，Michel Lequesen index score and serum cytokine levels of the 2 groups before and after the treatment were compared. Results:The total effective rate of the observation group was 97.71%，which was higher than 89.31% of the control group(P<0.05). After treatment the total score of TCM symptoms，VAS score，WOMAC total score，Michel Lequesen index score and the levels of malondialdehyde(MDA)，interleukin-1(IL-1)and interleukin-6(IL-6)in the 2 groups were significantly lower than before. Those of the observation group was lower than that of the control group(P<0.05). After treatment，the levels of superoxide dismutase(SOD)，transforming growth factor-β(TGF-β)and fibroblast growth factor-2(FGF-2)in the 2 groups were significantly higher than before. Those of the observation group was higher than that of the control group(P<0.05). The incidence of adverse reactions in the observation group was 0.76%，which was lower than 6.87% of control group(P<0.05). Conclusion:Bushen Huoxue recipe has significant effects on KOA patients with kidney deficiency and blood stasis syndrome，which can improve the clinical symptoms and signs，reduce the level of inflammatory factors. Furthermore，it has little toxic side effects.