To investigate the effects of dialectical treatment of diabetic nephropathy renal insufficiency on protein non-enzymatic glycosylation and protein kinase C activation and its relationship with hemodynamic abnormalities，and provide reference for clinical treatment of patients with diabetic nephropathy renal insufficiency.Methods:A total of 96 patients with renal insufficiency due to diabetic nephropathy were admitted to Puyang Traditional Chinese Medicine Hospital from March 2016 to March 2018 and they were randomly divided into observation group and control group，with 48 cases in each group.The control group was given routine Western medicine treatment，and the patients in the observation group were treated with the dialectical treatment of traditional Chinese medicine (TCM) based on the above-mentioned treatment.The renal function，protein kinase C (PKC) activity，and changes in blood glucose and glycosylated hemoglobin levels before and after treatment were compared between the two groups.The changes of blood flow related parameters after treatment in the two groups of patients were analyzed to observe the clinical efficacy of the two groups after treatment.Results:There was no significant difference between the two groups in terms of parameters related to renal function before treatment，PKC activity，and blood glucose and glycosylated hemoglobin levels (P>0.05).After treatment，the SCr，BUN，cytoplasmic PKC activity，membrane PKC activity，fasting blood glucose，and hemoglobin A1c of the patients in the observation group significantly decreased，and Ccr levels significantly increased，which was statistically significant (P<0.05).Compared with the control group，Vsmax of MRA and SRA in the observation group had no significant difference (P>0.05)，and Vdmin increased significantly，RI decreased significantly (P<0.05)，while Vdmin，Vsmax of IRA increased significantly and RI decreased significantly (P<0.05).The effective rate in the observation group was 95.83%，which was significantly higher than that in the control group (70.83%).The difference was statistically significant (P<0.05).Conclusion:The dialectical treatment of diabetic nephropathy patients with renal insufficiency can effectively inhibit the activation of PKC pathway，reduce protein non-enzymatic glycosylation，correct renal hemodynamic abnormalities，improve renal function，the effect is significant.