To establish an HPLC methods for free，portion and total bilirubin in Xiaoer Huadu Capsule(Powder）and compare the difference between 2 types of Calculus Bovis.Methods:The free，portion and total bilirubin were extracted with dichloromethane，10% oxalic acid solution(containing 0.15% cetyltrimethylammonium chloride)-dichloromethane，0.2 mol/L disodium ethylenediaminetetraacetate-dichloromethane，determined by HPLC with C18 column，the mobile phase was acetonitrile-1%glacial acetic acid(95:5).The detection wavelength is 450 nm，and the column temperature was 25 ℃.The flow rate was 1.0 mL/min.Results:Bilirubin showed good linear relationship in the range of 5.64 ng-282 ng，and the average recoveries of free，portion and total bilirubin was 90%-95%，with RSD 4.2%，4.1% and 4.9%.There were significant differences in the content and form of bilirubin of 2 kinds of Calculus Bovis.Conclusion:The method established is easy to operate with high effectiveness in identifying bezoar in Xiaoer Huadu Jiaonang and other Chinese traditional patent medicines containing Calculus Bovis.