To study comprehensive screening multi-components in Radix Astragali and Huangqi injection，based on high-resolution quadrupole-time-of-flight mass spectrometry.Methods:The analysis was performed by Agilent Eclipse Plus C18 column(3 mm × 100 mm，1.8 μm）with the mixture of 0.1% formic acid aqueous solution-acetonitrile as mobile phase by gradient elution，and the flow rate was 0.4 mL/min.Q-TOF-MS equipped with ESI ion source was performed in both positive and negative ionization.Samples were diluted with methanol.Qualitative analyze was based on retention time，the accurate mass of the precursor ion and product ions.Results:A total of 46 components including amino acids，flavonoids，saponins，nucleosides and nicotinic acid were successfully identified or tentatively characterized.Conclusion:This study is expected to provide an effective pattern for systematic characterization of the complex Huangqi injection systems，and establish a reliable strategy for its quality evaluation，also offers a reference for quality control as well as technological improvement.