To develop a sensitive and accurate method combining liquid and solid phase extraction with ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry to analyze 27 dyes.Methods:Different preparations，chromatography conditions and polarity of ESI were applied for 3 categories of lipophilic，acidic and basic dyes respectively.Four species of herbs representing different parts of plant were selected for methodology observation.Results:The linear correlation coefficient was good(r ≥ 0.997).The LODs of oil soluble and basic dyes were 0.03～3.87 ng/g and acid dyes were 0.003～1.94 μg/g respectively.The accuracies and precisions were 70%～105% and 0.9%～6.8% respectively.Conclusion:After detecting 80 samples of 25 species herbs，this method proved to be reliable，efficient and accurate in the detection of different kinds of dyes in the complex matrix of herbs.