To establish a method for the identification of colla corii asini in donkey-hide gelatin cake and the detection of constituents from horse，ox，sheep or pig skin with characteristic peptide as the detection marker.Methods:The protein was extracted by trichloroacetic acid precipitation method，and trypsin was used for enzymatic hydrolysis.UHPLC-QQQ MS was used with electrospray positive ionization mode and multiple reaction monitoring(MRM）to detect the target ions.Results:The method was validated to be specific and sensitive.The results showed that some samples were found to be containing colla corii asini and some samples containing bovine skin ingredients，whereas some samples did not detect any skin-derived constituents.Conclusion:The established method was validated to be simple and specific.It can be used for identification of colla corii asini in donkey-hide gelatin cake and detection of adulteration.