To explore the effects of different intensity on the efficacy of Gua Sha，the working mechanism and the effective period of Gua Sha preliminarily，and to further explore the best time interval between two Gua Sha treatments.Methods:The parameters such as the frequency of Gua Sha were controlled by the self-made special Gua Sha plate，and Gua Sha was respectively performed with the force of 1N，3N and 9N.The changes of IL-1α and IL-6 after Gua Sha were detected by ELISA.The effects of Gua Sha with different intensity on the body was tested via the method of skin HE staining，and the appropriate Gua Sha intensity was selected.The changes of leukocyte count，lymphocyte count and T cell subpopulations at different time points after Gua Sha were detected by routine blood examination and flow cytometry.The working mechanism and effective period of Gua Sha were preliminarily discussed.Results:The Gua Sha of 1N intensity did not cause obvious changes，while Gua Sha of 9N intensity caused relatively serious skin injury and the release of a large number of inflammatory factors.Gua Sha of 3N intensity was of moderation，which caused moderate stress reaction of the body.After Gua Sha with 3N intensity，the total number of leukocytes and lymphocytes，IL-1α and IL-6 increased slowly，reached the peak between 1 hour and 24 hours，then decreased slowly，and returned to normal level at 72 h.Conclusion:3N intensity is suitable for Gua Sha experiments.In between 1 hour and 24 hours after Gua Sha，the boosting effect of Gua Sha on the immune system peaked，which returned normal at 72 hours after the performance，suggesting the proceeding of the next treatment.