To establish the quality standard of Yangnao Ningxin Granules.Methods：TLC method was used to qualitatively identify the Cortex Moutan Radicis，Fructus Corni and Fructus Lycii in the prescription.The content of puerarin in peony was determined by HPLC.Results：In the thin-layer chromatogram，the corresponding spots of the corresponding color of the Cortex Moutan Radicis，Fructus Corni and Fructus Lycii spots correspond to the same color; the puerarin injection volume was 0.23～1.15 μg，showing a good linear relationship(r=0.999 9)，average recovery：98.5%，RSD 0.81%.Conclusion：The method is simple，easy to repeat，and can be used as a quality control standard for the preparation.