On the basis of clarifying the development of zang-fu syndrome differentiation and defining the connotation characteristics of zang-fu syndrome differentiation theory in different periods，to further explain the beneficial supplement and relevant contributions made by Zhang Yuansu to the improvement of zang-fu syndrome differentiation theory.Methods：Through tracing method in medical research method，literature of traditional Chinese medicine and TCM philology research methods，the pre-Qin period，Jin，Sui，Tang，Song Dynasties，Jin and Yuan Dynasties about differentiation of ancient books and the expoundation were collected and organized，and combined with modern literature retrieval method to supplement，clarify the differentiation of development context，summarizes the development of syndrome differentiation of zang-fu by Zhang Yuansu and Yi Shui school.Results：The Medical Origin written by Zhang Yuansu filled in the theoretical blank of the theory system of zang-fu syndrome differentiation，enriches the connotation of zang-fu syndrome differentiation theory from relevant academic thoughts and clinical application，and extended the vitality of zang-fu syndrome differentiation in the long river of traditional Chinese medicine.Conclusion：Zhang Yuansu is the completion of the theoretical system of zang-fu syndrome differentiation，and more importantly，he enriches and extends the vitality of zang-fu syndrome differentiation in the application of clinical syndrome differentiation.