To observe the clinical efficacy of an empirical formula of Yangjing Decoction combined with ecobalamine tablets to improve the neuroelectrophysiology in patients with diabetic peripheral neuropathy of yang deficiency,cold coagulation and collateral stasis,and to explore the mechanism of the treatment of diabetic peripheral neuropathy.Methods：A total of 110 cases of diabetic peripheral neuropathy admitted in Qionghai Hospital of Traditional Chinese Medicine from February 2017 to February 2018 were selected as research subjects and treated,and they were randomly divided into control group and obserbation group,with 55 cases in each group.The control group was given oral mecobalamine tablets.On the treatment basis of the control group,the observation group was given the empirical formula of Yangjing Decoction orally.The treatment course lasted for a month.Clinical efficacy of the 2 groups of patients and Toronto clinical scoring system(TCSS）scores before and after the treatment were compared.Improvements of nerve conduction velocity in the 2 groups of patients before and after the treatment were observed.Levels of hypoxia-inducible factor-1α(HIF-1α),soluble vascular cell adhesion molecule-1(sVCAM-1）and myelin basic protein(MBP）were monitored.Results：The effective rate of the observation group was 90.90%,significantly higher than 76.36% in the control group(P<0.05).After the treatment,the nerve conduction velocity of the 2 groups of patients was improved compared with that before the treatment.In the observation group,the nerve conduction velocity of the median nerve and the nerve conduction velocity of common peroneal nerve,SNCV and MNCV,were significantly higher than those of the control group(P<0.05).The TCSS scores of the 2 groups were significantly lower than those before the treatment,and the TCSS score(4.02±1.80）of the observation group was significantly lower than that of the control group(7.28±1.42)(P<0.05).Peripheral blood HIF-1,sVCAM-1,MBP levels of the 2 groups were significantly lower than those of the control group(P<0.05).Conclusion：The empirical formula of Yangjing Decoction can improve the nerve conduction velocity of patients with diabetic peripheral neuropathy of yang deficiency,cold coagulation and collaterals stasis,and has prominent advantages in improving symptoms.Its efficacy may be related to that it can protect the nerve by intervening the levels of HIF-1α,sVCAM-1 and MBP in peripheral blood to reduce the lesions of endothelial cells in nerve microvessels.