To study the therapeutic effect and possible mechanism of Rhizoma Sparganii-Rhizoma Sparganii in the treatment of uterine leiomyoma.Methods:A total of 36 female SD rats were randomly divided into a blank group,a model group,a 1：1 group and a 1：2 group,with 9 rats in each group.The model group,1：1 group and 1：2 group were used to prepare uterine myoma model.The blank group and the model group were given normal saline by gastric lavage.The 1：1 group and 1：2 group were given gastric lavage according to the compatibility ratio of trigonum and zedoary turmeric.After intervention,the changes of uterus/ovary coefficient,uterine size,uterine pathological morphology and the expression of PI3K/AKT pathway marker factors were compared in each group.Results:1)The uterine smooth muscle in the model group showed obvious pathological changes,and the uterine tissue lesions improved after the intervention of Rhizoma Sparganii-Rhizoma Sparganii,and the improvement in the 1：1 group was more obvious than that in the 1：2 group.2)The uterine coefficient,ovarian coefficient,transverse and vertical diameter of uterus,and the expression levels of E2,ER,PI3K and p-AKT/AKT in uterine tissue of rats with uterine leiomyoma significantly increased(P<0.05).The above indexes decreased after intervention with Rhizoma Sparganii-Rhizoma Sparganii.The improvement in 1：1 group was more obvious than that in 1：2 group(P<0.05).Conclusion:The compatibility of Rhizoma Sparganii-Rhizoma Sparganii can improve uterine leiomyoma,and this mechanism may be achieved by activating PI3K/AKT pathway.