To establish an LC-MS/MS method for determination of dendrobine, and to study the pharmacokinetics of dendrobine in rats.Methods:Six male rats were orally administered with Dendrobium(20 mg/kg),Plasma samples were collected at 5、15、30、45、60、90.120、180、240、420、600、1440 min after the drug administration.The pharmacokinetic parameters were calculated by DAS2.0 software.Results:The calibration curves were linear over the concentration ranging from 0.5 to 50 ng/mL（r2=0.9992）with the LLOQ of 0.5 ng/mL.The intraday and interday RSD were generally good(<10%).The concentration-time curve for dendrobine was in accordance with the two-compartment model, The main pharmacokinetic parameters t1/2, Tmax, Cmax, AUC(0-∞)were as follows:(51.27±2.65)min,(10.00±0.17)min,(0.12±0.08)mg/L, （7.91±0.25）mg/L·min.Conclusion:The analytical method developed is sensitive specific rapid and reproducible and is proved to be suitable for the pharmacokinetic study of dendrobine.