To investigate the efficacy of self-made compound Zuogui Capsule Combined with acupuncture sequential therapy in the treatment of DOR patients and its effect on the levels of BDNF，TAC and quality of life in follicular fluid.Methods:A total of 124 patients with DOR admitted to Puren Hospital Affiliated to Wuhan University of Science and Technology from August 2017 to August 2017 were selected as the research subjects.They were divided into a control group and an observation group by random number grouping method，with 62 cases in each group.All patients were treated with estradiol valerate+medroxyprogesterone acetate，and the control group was treated with self-made compound Zuogui Capsule on the basis of this.The observation group was treated with acupuncture sequential therapy on the basis of the control group.The clinical effects of the 2 groups and the changes of FSH，E2，BDNF，TAC and quality of life before and after treatment were compared.Results:There was no significant difference in the level of FSH，E2，BDNF and TAC，as well as the quality of life before treatment(P>0.05).The levels of FSH，E2，BDNF and TAC decreased significantly in the 2 groups，and the scores of body，role，emotion and social function were significantly increased(P>0.05).After treatment，the levels of FSH，E2，BDNF and TAC in the observation group were lower than those in the control group，and the scores of body，role，mood and social function were higher than those in the control group(P<0.05).The cure rate of the observation group was 40.32%(25/62）and the total effective rate was 93.55%(58/62)，which were significantly higher than that of the control group 22.58%(14/62）and total effective rate 79.03%(49/62)(P<0.05).There was no significant difference in the incidence of adverse reactions between the 2 groups(11.29%：9.68%)(P>0.05).Conclusion:Compound Zuogui Capsule combined with acupuncture sequential therapy can significantly improve the levels of serum FSH，E2，BDNF and TAC in the serum of DOR patients，and improve the quality of life.The clinical effect is remarkable.It is worthy of further application.