To compare the fingerprints of traditional decocting method and modern decocting machine methods of Sanhuang Decoction by high performance liquid chromatography(HPLC).Methods:Ten different batches of Sanhuang Decoction were randomly selected by random digital table method to prepare Sanhuang decoction solution of traditional decocting method and Sanhuang Decoction solution of modern decocting machine.The chromatographic conditions were as follows：DIAMONSIL C18 protective column(5 μm）of 4.6 mm×250 mm，column temperature 30 ℃，injection volume 15 μL，methyl alcohol as mobile phase A and 1% phosphoric acid water as mobile phase B，detection wavelength of 277 nm，for HPLC fingerprint comparison.Results:1）The specificity of Sanhuang Decoction was tested by HPLC.The results showed that the specificity of Sanhuang Decoction was good. 2） Linear relationship test showed that the regression equation of baicalin y=75.64X+21.31，r=0.999 8(n＝5).The results showed that the range of baicalin reference substance was 0.5~12.0 ug/mL，and the linear relationship was good. 3）The RSD average value of relative retention time of chromatographic peaks larger than 5% of the total peak area was 1.776%.The results showed that the solution of Sanhuang Decoction had good precision. 4）The results of stability showed that the RSD value of relative retention time of chromatographic peaks was 1.843%，suggesting that the solution of Sanhuang Decoction had good stability within 24 hours. 5）The results of repeatability test showed that the RSD value of relative retention time of chromatographic peaks was 1.911%，suggesting that the repeatability of Sanhuang Decoction was good. 6）The average recovery was 99.81% and RSD was 1.79%.The results showed that the HPLC was reliable. 7）Sample determination results showed that under the same chromatographic elution gradient，the fingerprint of traditional decocting method of Sanhuang Decotion was completed within 120 minutes，and the fingerprint of modern decocting machine prepared Sanhuang Decotion was completed within 131.87 minutes; In addition，from the chromatogram，it can be seen that there were more chromatographic peaks in the modern decocting machine prepared Sanhuang Decoction，which may be compared with that more common peaks can be separated from the high pressure decocting equipment of the modern decocting machine.From the similarity of fingerprints，the similarity of 10 batches of Sanhuang Decoction prepared by traditional decocting method and 10 batches of Sanhuang Decoction prepared by modern decocting machine method were higher than 0.9，of which the average similarity of traditional decocting method was 0.9714，and that of modern decocting machine method was 0.9816，slightly higher than that of traditional decocting method.Conclusion:The HPLC fingerprint of Sanhuang Decoction prepared by traditional decocting method and modern decocting machine method is different.