To study the regulatory effect of penetration enhancers Azone and borneol applied with herbal cake-partitioned moxibustion on blood lipid and inflammatory factor levels，and to observe the regulatory effects on the levels of blood lipids and inflammatory factors of a hyperlipidemia rabbit model.Methods:A total of 40 New Zealand white rabbits were randomly divided into a normal group，a hyperlipidemia group，a moxibustion treated only hyperlipidemia group，an Azone plus moxibustion treated hyperlipidemia group and a borneol plus moxibustion treated hyperlipidemia group，with 8 rabbits each.The normal group rabbits were feed normally，whereas other rabbits were firstly treated with high-fat food for 12 weeks to generate hyperlipidemia model，followed by treatment of herbal cake-partitioned moxibustion applied with penetration enhancers for 4 weeks.Afterwards，rabbit serum was collected and measured for levels of total cholesterol(TC)，tryglyceride(TG)，high-density lipoprotein cholesterol(HDL-C)，low-density lipoprotein cholesterol(LDL-C)，TNF-α，and INF-γ.Results:All the 3 herbal cake-partitioned moxibustion groups significantly reduced blood TC，TG and LDL-C(P<0.05)，and significantly increased HDL-L(P<0.05).The effect of the Azone group decreasing TC and TG，and the borneol group decreasing blood TC，TG and LDL-C levels was better than no penetration enhancers group(P<0.05).The borneol group was better than the Azone group in reducing TC(P<0.05).The 3 herbal cake-partitioned moxibustion groups significantly reduced blood TNF-α，and INF-γ levels(P<0.05)，and the Azone group and the borneol group were better than no penetration enhancers group(P<0.05).The borneol group was better than the Azone group in reducing TNF-α(P<0.05).Conclusion:Herbal cake-partitioned moxibustion can effectively regulate the blood lipid and inflammatory factors in the high-fat model rabbits.The application of Azone and borneol，as a penetration enhancer，have more significant regulatory effect on the levels of blood lipids and inflammatory factors of a hyperlipidemia rabbit model after herbal cake-partitioned moxibustion，and the Azone group was better than the borneol group，which deserves further study.