To establish a rat model of post-traumatic stress disorder(PTSD)through single-prolonged stress(SPS)and to observe the effect of Zhenjing Wendan Decoction on the behavior of the SPS model rats.Methods:A total of 40 rats were randomly divided into a normal group，a model group，a Zhenjing Wendan Decoction group and a Paroxetine group.The rats in the last 3 groups were modeled by SPS.And then the rags were receiving gavage for 7 days.After that，Open Field Test and Freezing Time Test were performed.Results:Compared with the model group，all groups except the normal group had significant differences in several indicators，such as the total distance of movement，maximum distance of movement and the number of grid penetration，especially in freezing time(P<0.05).Among them，Zhenjing Wendan Decoction had significant difference，making the indicators of maximum distance of movement，erect times，and single movement distance better than the Paroxetine group，but there was no significant difference in freezing time.Conclusion:This study succeeded repairing PTSD model rats through SPS.Zhenjin Wendan improved the anxiety and fear state of SPS model.