To investigate the effects of berberine combined with Peikun pill on lipid and glucose metabolism，inflammatory factors and hormone levels in patients with insulin resistance of polycystic ovary syndrome(PCOS-IR).Methods:A total of 96 patients with PCOS-IR who were treated in Xiaolan Hospital Affiliated to Southern Medical University from March 2016 to March 2017 were selected as research subjects.The patients were divided into a control group and an observation group according to random number method，with 48 cases in each group.The control group was treated with metformin，and the observation group was treated with berberine combined with Peikun pills.The clinical efficacy，lipid and glucose metabolism，inflammatory factors and hormone levels of the 2 groups were analyzed.Results:The total effective rate of the observation group was 93.75%，which was not significantly different from the 89.58% of the control group(P>0.05).After treatment，the total cholesterol(TC)，triglyceride(TG)，low density lipoprotein cholesterol(LDL-C）of the 2 groups were all lower than those before treatment(P<0.05)，but high density lipoprotein cholesterol(HDL-C）had no significant change.After treatment，the fasting insulin(FINS）and insulin resistance index(HOMA-IR）of the observation group were lower than those before treatment，and the fasting blood glucose(FBG）and HOMA-IR of the control group were lower than before treatment(P<0.05).The levels of serum C-reactive protein(CRP)，interleukin-6(IL-6）and tumor necrosis factor-α(TNF-α）in both groups were lower than those before treatment，and the observation group were lower than those in the control group(P<0.05).There was no significant difference in follicle stimulating hormone(FSH）between the 2 groups before and after treatment(P>0.05).After treatment，the luteinizing hormone(LH)，testosterone(T）and LH/FSH of the 2 groups were all lower than those before treatment.The LH and LH/FSH in the observation group were lower than those in the control group(P<0.05).Conclusion:Berberine combined with Peikun pill can effectively treat PCOS-IR，improve lipid and glucose metabolism，inflammatory factors and hormone levels in patients.The clinical efficacy is comparable to metformin，but the improvement of inflammatory factors and hormone levels is more obvious.