To investigate the therapeutic effects of the method of activating blood circulation，resolving blood stasis，softening and resolving hard mass in treating endometriosis，and observe its effects on humoral immune factors.Methods:A total of 90 patients with endometriosis admitted to The First Clinical Medical College of Nanjing University of Chinese Medicine from June to December 2017 were selected and randomly divided into an observation group and a control group，with 45 cases in each group.The control group was treated with gestrinone capsules orally.On the basis of this，the observation group was given activating blood circulation，resolving blood stasis，softening and resolving hard mass，and continued treatment for 6 months.The clinical efficacy，hemorheological index as well as immunoglobulin(IgA，IgG，IgM）and complement(C3）levels of the 2 groups were observed.Results:After treatment，the scores of TCM symptom scores(6.05±2.21）and dysmenorrhea symptom scores(3.81±1.03）in the observation group were significantly decreased than those in the control group [(7.56±2.57)，(4.56±1.27)](P<0.05); the whole blood high-viscosity，whole blood low-cut viscosity，plasma specific viscosity，erythrocyte rigidity index and erythrocyte aggregation index of the observation group were significantly lower than those of the control group(P<0.05); IgA(1.38±0.16）g/L，IgG(4.89±0.56）g/L，IgM(1.14±0.18）g/L and C3(1.35±0.22）g/L in the observation group decreased when compared with that before treatment，and the degree of decline was significantly higher than the control group(P<0.05); the total effective rate of the observation group was as high as 93.75%，while the control group was only 79.17%.The difference was statistically significant(P<0.05).Conclusion:The treatment of endometriosis with the method of activating blood circulation，resolving blood stasis，softening and resolving hard mass can effectively alleviate the symptoms of patients and regulate humoral immunity.The effect is significant and it is worth further promotion and application.