To establish a UPLC-MS/MS method for the simultaneous determination of 7 kinds of boswellic acids in Olibanum，and compare the contents in samples from different origins.Methods:The determination was performed on the ACQUITY UPLC BEH C18(2.1 mm × 100 mm，1.8 μm); the 0.1% formic acid-water(A)and 0.1% formic acid-acetonitrile(B)in gradient elution as mobile phase.The flow rate was 0.5 mL/min.The column temperature was 40 ℃.MS instrument was equipped with ESI+ ion source.Gaining the extracted ion chromatograms，then the peak area was used for quantitative.Results:The 7 kinds of boswellic acids：α-boswellic acid，β-boswellic acid，3-acetyl-α-boswellic acid，3-acetyl-β-boswellic acid，11-keto-β-boswellic acid，acetyl-11-keto-β-boswellic acid and 3-keto-11α-hydroxy-β-boswellic acid had good linearity，r>0.9985.The recovery rate was 96.8%～102.0%，with RSD<3.5%.Conclusion:The method is simple，accurate and repeatable，and provides a reference for in-depth research and quality control of boswellic acids in mastic.