To analyze the molecular biological mechanism and related pathways of spinal cord injury by tanshinone IIA based on network pharmacology.Methods:The target sites of tanshinone IIA were screened by TCMSP，BATMAN-TCM，DTPS，STITCHI and Swiss Target Prediction.The genes related to spinal cord injury were searched by OMIM，GeneCards and CTD databases，and the target of the spinal cord injury was screened by Wayne map.The STRING database was used for protein interaction network analysis.The protein interaction network was constructed by Cytoscape software，and the Metascape database was used for GO analysis and KEGG signal pathway enrichment analysis.Results:Tanshinone IIA had a total of 182 potential targets and 157 targets related to spinal cord injury，including tumor protein p53(TP53)，Myc proto-oncogene protein(MYC)，and G1/S-specific cyclin-D1(CCND1).The 5 targets of caspase 3(CASP3)and JUN proto-oncogene protein(JUN)played an important role in the protein interaction network.The biological processes involved in the treatment of spinal cord injury by tanshinone IIA mainly include drug reaction，lipopolysaccharide reaction，and cell response to DNA damage stimulation; the molecular functions involved include enzyme binding，transcription factor binding，and protein binding.Its mechanism may be related to cAMP signaling pathway，MAPK signaling pathway，PI3K-Akt signaling pathway，HIF-1 signaling pathway，TNF signaling pathway，apoptosis and other pathways.Conclusion:Tanshinone IIA has multiple target and multi-channel effects in the treatment of spinal cord injury.It may interfere with the process of spinal cord injury by promoting neuronal survival，promoting axon regeneration，inhibiting neuronal apoptosis and anti-inflammatory.