To observe the differences of SD rat asthma models with different methods of sensitization fluid composition, sensitized injection site, atomization liquid concentration and time, aiming to establish a more successful asthma rat model. Methods:The10% OVA/Al(OH)3 mixture 1 mL or 10% OVA/Al(OH)3+ pertussis mixture 1 mL were taken as the sensitization fluid respectively; sensitized injection site was abdominal injection or 5 points injection (bilateral foot heart, bilateral inguinal subcutaneous and abdominal injection); using 1% OVA or 2% OVA as the excitation liquid; excitation time including 7 days and 21 days. General activities, pulmonary pathology and pulmonary function detection were evaluated by different combination methods of factors above. Results:Typical asthmatic symptoms such as shortness of breath, dyspnea and so on in OVA+ pertussis abdominal injection group and OVA+ pertussis 5 points injection group rats were obvious. Mild asthma symptoms were observed in the first OVA group rats, and asthma symptoms in other groups were not obvious. The pathological results showed that OVA+ pertussis 5 points injection group rats showed a large number of eosinophils and lymphocytes infiltration, bronchostenosis, mucus production and bronchospasm. The first OVA group and the OVA+ pertussis abdominal injection group rats showed infiltration of lymphocytes and goblet cells. The pathological changes in the other groups were not obvious. Results of lung function showed that: I and Rn values of OVA+ pertussis 5 points injection group and OVA+ pertussis abdominal injection group rats increased compared with those of the OVA abdominal injection group. Hyperresponsiveness of OVA+ pertussis 5 points injection group increased more significantly (P<0.01). Conclusion:Sensitization with both OVA and pertussis, multipoint subcutaneous injections combined with abdominal injection can successfully construct the asthma model in SD rats.