By using the acute toxicity test of mice and the comprehensive toxicity evaluation technology based on Microtox technology, to compare the toxicity of ethanol extracts from Honeysuckle and Lonicera Japonica, and to explore the feasibility of Microtox technology in rapid toxicity evaluation of traditional Chinese medicine. Methods:Mice were given ethanol extracts from Honeysuckle and Lonicera Japonica at different concentrations in a single gavage, and the toxicity reaction and death of the mice were observed and recorded, then calculated the median lethal dose(LD50)of the mice. The inhibition rate of ethanol extracts from Honeysuckle and Lonicera Japonica to the luminescence intensity of the luminescent bacteria was measured by Microtox technology, and calculated the half inhibitory concentration(IC50)of the luminescent bacteria. The results of the two toxicity evaluation methods were compared. Results:The median lethal dose(LD50)values of the ethanol extract from Honeysuckle and Lonicera Japonica were respectively crude drug 81.29 g/kg and crude drug 91.00 g/kg. The half-inhibition rate of luminescent bacteria measured by the bio-complex toxicity evaluation technique was crude drug 0.0013 g/mL and crude drug 0.0022 g/mL, respectively. Conclusion:The acute toxicity test in mice and the comprehensive toxicity test based on Microtox technology were used to test the extracts of Honeysuckle and Lonicera Japonica. The two kinds of evaluation results have a certain consistency. It is feasible to use Microtox technology to evaluate the toxicity of extracts from Honeysuckle and Lonicera Japonica, but the correlation between the two methods needs to be further studied with a larger sample size.