To build root quality standard of Chuan Danshen(Salviamiltiorrhiza). Methods:Based on root length, root thickness, hundred root weight, purity, clarity and water content, the K-class clustering method was used to grade root level of Chuan Danshen, and field cultivation was carried out for grade verification. Results:The roots of Chuan Danshen were divided into three grades, first grade:root thickness>13 mm, 100 roots weight>2 500 g, clarity ≥ 95%; second grade:root thickness:7~13 mm, 100 roots weight:2 000~2 500 g, clarity ≥ 90%; third grade:root thickness<7 mm, 100 roots weight<2 000 g, clarity<90%. Conclusion:The established root grading standard of Chuan Danshen can provide scientific standard for original root selection of Chuan Danshen.