To observe effects of Chaihu Plus Longgu Muli Decoction on serum ACTH and CORT in mice with acute myocardial infarction(AMI)complicated with anxiety.Methods:Thirty mice were randomly divided into sham operation group, AMI group, anxiety group, AMI+anxiety group and traditional Chinese medicine group(n=6).The compound model was made through coronary artery ligature combined with empty bottle stimulation and intervened by Chaihu Plus Longgu Muli Decoction for 14 d.After the establishment of the model, behavioral and Echocardiography detection was conducted.After execution, contents of ACTH and CORT in the serum in each group were detected.Results:Compared with the sham operation group, LVEF and LVFS in the AMI group were significantly decreased, while LVEDd and LVESd were significantly increased.Scores of horizontal and vertical movements in the AMI group and the anxiety group were decreased, while open arm entry times and residence time were decreased.The ACTH and CORT contents were increased in the anxiety group, and only the ACTH content was increased in the AMI group.Compared with the AMI+anxiety group, the LVEF and LVFS in the AMI, anxiety and traditional Chinese medicine groups were significantly increased, while the LVEDd values were significantly decreased; the horizontal and vertical movement scores were increased; and the serum ACTH and CORT contents were decreased.The LVESd values were decreased only in the AMI and anxiety groups.OE% was increased and OT% was prolonged in the AMI and traditional Chinese medicine groups, and only OT% was prolonged in the anxiety group.Conclusion:The therapeutic mechanism of Chaihu Plus Longgu Muli Decoction in anti-anxiety and improving the prognosis of AMI may be related to the inhibition of the increase of ACTH and CORT contents.