Acupuncture manipulation (MA) is a key factor to acupuncture effect. However, the effect regularity and mechanism of MA are not clear. Professor GUO Yi's team in Research Center of Experimental Acupuncture Science of Tianjin University of traditional Chinese medicine and Professor WANG Jiang's team of School of Electrical and Information Engineering of Tianjin University have carried out cooperative research on the manipulation effect regularity and neural electric information mechanism of MA for a long time. The mathematical model of commonly used MA was established to depict the operation process of MA with mathematical language, which realized scientific representation of acupuncture. Part of the regularity of MA based on neural electric information has been revealed. The nonlinear dynamic characteristics of electric signals evoked by MA was described, and the differences of neural electric information coding induced by various MA was confirmed, which is one of the mechanisms of MA with different effects. These research results provide experimental basis for the scientificity of MA and promote the inheritance, application and development of MA.