How the acupuncture effects is initiated is a common key issue in the study of the acupuncture action principle.For many years，under the affirmative acupuncture effects，starting with the local chemical ions at the acupoints，from dots to lines，and from lines to nets，the research was conducted from different angles of nerve，immunity，endocrine，and energy metabolism，exploring local nerve-cell-chemical interaction and systematically studying “Acupuncture Network” changes and its relationship with acupuncture effects.It has been proved that: 1）Acupuncture could excite nerves that innervating acupoints thus initiate neuroregulation. 2)Acupuncture could induce local inflammatory reaction of acupoint and initiate immune regulation. 3)Acupuncture could regulate the levels of some hormones in acupoint area. 4)Acupuncture could regulate blood flow and partial pressure of oxygen in acupoint area，thus affecting local energy metabolism. 5)The post-interaction of nerve-mast cell-chemical substances in acupoint area influences acupuncture effects. 6)The directed weighting model and the complex network model are supposed to describe the time series response characteristics of the initiation regulatory network of acupuncture，and locate the key signaling molecules.In the study of the initiation mechanism of acupuncture effects，we should combine the local reaction with the whole effects，establish the interactional，mutually connected concept of complex network.And based on systematic summarization and abstraction，we uncovered the key basic factors of acupuncture，acquiring its effective regulation mode and finally improving its clinical efficacy of acupuncture.