Biosensor technology based on surface plasmon resonance (SPR) phenomenon has been widely concerned and applied in recent years because of its advantages，such as no labeling，sensitivity，high-throughput，specificity，low sample consumption，etc.In the study of the mechanism of traditional Chinese medicine (TCM)，SPR biosensor technology has also been used in many fields in recent years.In this paper，the principle of SPR is briefly introduced.The research progress and prospect in the fields，including the new target discovery of TCM and searching for small molecules with best performance，are summarized and reviewed.