To study the effects of Qizhu Kangai Formula on the proliferation and apoptosis of HepG2 heptoma cells of liver cancer.Methods:The HepG2 cells were cultured in vitro and treated with different concentration of Qizhu Kangai Formula (0,250,500,1 000 mg/L ) for 48 h.The living cells were detected by cell counting kit 8 ( CCK8 ) and the protein expression of c-caspase3 and c-caspase9 were observed by Western-blot.Results:CCK8 showed that Qizhu Kangai Formula could inhibit HepG2 cells proliferation in concentration-dependent manner and had significant differences with the control group (P<0.05).Among them,the 1000 mg/L group had the best inhibition effects.Further studies found that the expression of the apoptotic proteins c-caspase3 and c-caspase9 increased after the stimulating HepG2 cells by Qizhu Kangai Formula and was concentration-dependent and was highest in the 1000 mg/L group with statistical significance ( c-caspase3 increased 1.83 times,P<0.05; c-caspase9 increases by 1.06 times,P<0.05 ).Conclusion:Qizhu Kangai Formula could inhibit the proliferation and induce apoptosis of HepG2 heptoma cells.