To improve the quality standard of Kangmin Granules.Methods:Sophora flavescens，Fructus Kochiae Scoparuae，Radix Grycyrrhizae and Radix Paeoniae Rubra were identified by thin-layer chromatography(TLC).The content of matrine in the preparation was determined by high performance liquid chromatography(HPLC).Chromatographic conditions:Agilent NH2 column(4.6 mm×250 mm，5 μm)，mobile phase conditions:acetonitrile-anhydrous ethanol-3% phosphoric acid(80:10:10)，detection at 220 nm wavelength，flow rate 1.0 mL/ min，column temperature 25 ℃，injection volume 10 L.Results:In the established TLC identification of Sophora flavescens，Radix Rhizoma，Radix Glycyrrhizae，and Radix Paeoniae Alba，the component spots were clear，the separation was good，and the negative control sample had no interference.The concentration of Sophora flavescens was in the range of 11.31~180.96 μg/mL，which has a good linear relationship with the peak area，R2=1; the average sample recovery rate was 100.88%，and the RSD was 2.31%(n=9).Conclusion:The method is accurate and easy to perform，with good precision，stability and repeatability，and can be used as a quality standard for Kangmin Granules.