To establish in-vitro administration method for Fuzheng Huayu(FZHY) and further to observe the medicinal effect differences of different batches, which will be fundermental for establishment of Traditional Chinese Medicine(TCM) bioactivity-based quality control. Methods:Human hepatocyte cell L-02, mouse leukemia cell of monocyte macrophage Raw264.7, mouse bone marrow-derived macrophage (BMDM) were selected to establish hepatocyte peroxidation injury model and macrophage activation model. Firstly, by investigating the influence of factors such as solvent, concentration and other factors on cellular effects, based on the best hepatoprotective and anti-inflammatory effects of Fuzheng Huayu in vitro medicine, an in vitro drug delivery method for Fuzheng Huayu extract was established; the effect of Fuzheng Huayu extract on liver cell damage and macrophage activation was different. Results:Fuzheng Huayu extract was dissolved in dimethyl sulfoxide and was not filtered. The chemical index components of different batches of Fuzheng Huayu are different, but there is no significant difference in overall cell biological activity. Among them, the biological activity of batch number 171005 was slightly less than that of other batches and was a little different. Conclusion:Hereby, standard in-vitro FZHY administration method was established. We found chemical component-based quality control is not consisted with its cellular bioactivity.