To explore the effects of Fuzi Lizhong Decoction on endotoxin and its receptor in rats with nonalcoholic steatohepatitis.Methods:A total of 75 SD male rats were randomly divided into 3 groups:a normal group，a model group and a treatment group with Fuzi Lizhong Decoction.Abdominal aortic blood sampling method was used，blood was collected and centrifuged to take supernatant，and the expression level of endotoxin was detected by double antibody filling method to detect tumor necrosis factor alpha(TNF-α)and Limulus synthetic matrix color method was used to detect endotoxin expression; immunohistochemical method was used to detect the expression level of CD14 in liver tissues，and the reverse transcription-polymerase chain reaction(RT-PCR)method was used to detect the expression levels of Toll-like receptor-4(TLR-4)and TNF-α mRNA.Results:Compared with the normal group，the level of TNF-α in the model group increased significantly，and continued to maintain a high level during the 12th to 24th weeks; the level of TNF-α in the Fuzi Lizhong Decoction was significantly lower than that in the model group，but it was still higher than that in the normal group.The expression of TNF-α mRNA in liver of normal group rats was not found，and the expression levels of TLR4 and TNF-α mRNA in model group were significantly increased.Compared with the model group，the TLR4 and TNF-α mRNA of the Fuzi Lizhong Decoction group were significantly lower than the model group，but it was still higher than TLR4 and TNF-α mRNA expression in the normal group; only a small number of cells in the normal group were able to express CD14.Compared with the normal group，the number of CD14-expressing positive cells in the model group's liver tissue continued to increase from 12 to 24 weeks，and slightly decreased at 24 weeks，while the number of CD14-expressing positive cells in the Fuzi Lizhong Decoction group significantly reduced compared with the model group.Conclusion:Fuzi Lizhong Decoction can reduce the expression of endotoxin and its receptor in non-alcoholic steatohepatitis rats.