To explore the mechanism of panax notoginseng saponins，total saponins of Astragalus combined with autologous bone marrow stem cell transplantation for treating diabetic ulcer on mirna-146a.Methods:A total of 60 SD rats were selected to make diabetic foot model on the dorsum of both feet.The fourth generation of bone marrow mesenchymal stem cells were isolated and expanded by whole bone marrow culture and adherent method.According random number table method，the rats were randomly divided into a blank group，a control group，a stem cell group，and Radix Astragali seu Hedysar group.The wounds of Radix Notoginseng，stem cell group，Radix Astragali seu Hedysar group were injected with autologous bone marrow stem cells(0.3 mL/CM2)every day，Radix Astragali seu Hedysar group was injected with astragalus injection(0.3 mL/CM2)every day，and Radix Notoginseng group was injected with Xuesaitong(0.4 mL/CM2)every day.After 7 days，they were sacrificed.The content of miRNA-146a was detected by PCR.The expression of TLR4 miRNA，NOD1 miRNA，and NF-κB miRNA was detected by Western blot.Results:Compared with the control group，the wound healing rate of rats in stem cell group，Radix Astragali seu Hedysar group and Radix Notoginseng group increased significantly(P<0.01)，and the expression of miRNA-146a，TLR4 miRNA，NF-κ B miRNA and NOD1 miRNA in stem cell group，Radix Astragali seu Hedysari group and Radix Notoginseng group increased significantly compared with the control group(P<0.05).Compared with stem cell group，the expression of miRNA-146a，TLR4 miRNA，NF-κ B miRNA and NOD1 miRNA in Radix Notoginseng group was significantly increased(P<0.05).Conclusion:Panax notoginseng saponins and astragalus saponins combined with bone marrow stem cells are effective in the treatment of diabetic foot ulcer，and the healing mechanism may be related to the up-regulation of miRNA-146a expression by TLR4/NF-κ B pathway.