To explore the target of Jianpi Jiedu Liangxue Formula in the treatment of intestinal barrier dysfunction in liver injury.Methods:A total of 32 male C57BL/6 mice were randomly divided into a normal group,a model group,a Chinese medicine group and a Peifikang group.Mouse liver injury model by intraperitoneal inject of thioacetamide 100 mg/kg was established.The mice in the traditional Chinese medicine group were given Jianpi Jiedu Liangxue Formula for gavage.The mice in the Peficon Kang group were given Pefikang solution.After16 hours,mice serum,liver tissue,and small intestinal tissue were collected.Liver function,intestinal HE histochemical changes,immunohistochemical staining method were observed the expression of ZO-1 protein in the small intestine.Results:The ALT and AST of the model group,the Chinese medicine group and the Peifikang group were significantly higher than those of the normal group（P>0.05).There was no significant difference between the model group,the Chinese medicine group and the Peifikang group.Small intestinal tissue can be observed under light and electron microscopy.Immunohistochemical staining in the cell membrane of intestinal villus epithelial cells were visible on the zonula occludens protein-1 Brown positive marker.The positive markers of model group,Chinese medicine group and Peifikang group were significantly less than which of normal group.The positive marker of Chinese medicine group were significantly more than that of model group（P<0.05).Conclusion:Jianpi Jiedu Liangxue Formula in the treatment of acute liver injury induced by thioacetamide can maintain the expression of zonula occludens protein-1 in the tight junction of intestinal epithelial cells and repair intestinal barrier function.