To explore the relationship between qi-deficiency syndrome of heart failure with preserved ejection fraction(HFpEF)with comorbidities and echocardiography.Methods:Using a cross-sectional study method，the information about four diagnostic methods of TCM and comorbidities in 165 HFpEF patients with qi-deficiency syndrome admitted by China-Japan Friendship Hospital from December 2017 to December 2018 were collected.According to the results of calculating scores of qi-deficiency syndrome，the patients were divided into qi-deficiency severe syndrome group(98 cases)and qi deficiency slight syndrome group(67 cases).And 60 patients without heart failure and qi-deficiency syndrome were enrolled as the control group.The measurement of Doppler echocardiography was finished in all patients.Results:The result of logistic regression analysis of qi-deficiency syndrome and comorbidities was that anemia ［OR＝0.214，95%CI(0.111 to 0.447)，P=0.000］，diabetes ［OR＝0.054，95%CI(0.006 to 0.113)，P=0.036］，chronic kidney disease ［OR＝0.057，95%CI(0.001 to 0.114)，P=0.046］ were risk factors for the formation of qi-deficiency syndrome in HFpEF patient.The correlation analysis between qi-deficiency syndrome and echocardiography indicators showed that the maximum speed(e')of the early dilation of the mitral annulus side was lower in qi-deficiency severe group than that in qi-deficiency slight group and control group(4.37 vs. 4.88 vs. 5.54，P=0.041，P=0.000)，and the early peak systolic velocity of mitral orifice dilation(E)/e' was highest(22.74 vs. 18.09 vs. 11.96，P=0.012，P=0.000)in qi-deficiency severe group.Conclusion:HFpEF qi-deficiency syndrome is associated with the distribution of comorbidities and impaired diastolic function.