The digestive tract is a tubular combination of multiple organs in the body,with complex composition,structure and function,and different characteristics in case of injury.The injury of digestive tract caused by drugs varies with the specific site,cell types,damage degree and marker types.Evaluation of tissue and cell marker enzyme activity is the simplest and most effective way to characterize the damage of the digestive tract.However,these markers are often changed due to metabolic disorders caused by in vivo underlying diseases,thus affecting the accuracy of injury assessment.Only by deeply understanding the injury mechanism can we achieve the accurate and stable evaluation of digestive tract injury.This paper reviews the composition and structure of digestive tract,and the impact of changes in injury markers induced by metabolic disorders on injury evaluation.