To explore the long-term high-fat feeding of ApoE-/-mice with TCM syndrome types，and provide animal models for the research of anti-atherosclerosis syndromes in traditional Chinese medicine.Methods:A total of 20 male ApoE-/-mice were divided into Simiao Yongan Decoction group (SM group) and model group (M group) according to the random number table method.10 mice in each group were fed with high-fat diet for 14 weeks to establish atherosclerosis model.Meanwhile，the SM group started preventive administration at the time of modeling.At the same time，10 mice of C57BL / 6 were used as the normal control group (group N).By measuring serum total cholesterol (TC)，triglyceride (TG)，high-density lipoprotein cholesterol (HDL-C)，and low-density lipoprotein cholesterol (LDL-C) levels，observing aorta and liver pathology to evaluate whether it was the blood stasis phlegm-dampness syndrome type; evaluating whether it was a poisonous syndrome type by the changes of IL-6 and NF-κB immunohistochemical.Results:Compared with the control group，the serum levels of TC，TG，and LDL-C in the model group were significantly increased (P<0.01)，HDL-C was significantly reduced (P<0.01); spherical lipid droplets and inflammatory cell infiltration appeared in the liver pathology; obvious plaques appeared in the aorta; aortic intima thickness (IT)，media thickness (MT)，plaque area (PA)，and IT / MT significantly increased (P<0.01)，and the vascular lumen area (LA) decreased significantly (P<0.01); immunohistochemistry showed that the expression of IL-6 and NF-κB in the aorta was significantly increased (P<0.05).Compared with the model group，the SM group can significantly reduce the aorta PA，IT and IT / MT of ApoE-/-mice (P<0.05)，and significantly reduce the expression of IL-6 and NF-κB (P<0.05); it also reduced liver disease but had no significant effect on blood lipid levels.Conclusion:The atherosclerosis model prepared by the classic long-term high-fat feed ApoE-/-mice was preliminary evaluated as an animal model of phlegm dampness and blood stasis and toxin damage.