To investigate the effects of Mahuang Lianqiao Chixiaodou Decoction on skin repair and expression of PAR-2 and TRPA1 in mice with atopic dermatitis(AD).Methods:A total of 48 SPF male BALB/c mice were randomly divided into a normal group，a model group，a hormone group and a traditional Chinese medicine group.Except for the normal group，AD models were induced by 2,4 dinitrofluorobenzene(DNFB).After modeling，the model group was given Piyanping external application，the traditional Chinese medicine group was given Mahuang Lianqiao Chixiaodou Decoction for intragastric administration，and the rest were given the same amount of distilled water intragastric administration，twice a day with a total of 7 days.On the 1st，15th，31st and 37th day，the general state and scratching behavior of mice were observed，and the pathological changes of sensitized areas were observed by HE staining.The gene and protein expressions of PAR-2 and TRPA1 in skin were detected by qRT-PCR and immunohistochemistry respectively.Results:Compared with the normal group，the scratching frequency of the model group mice increased significantly，and the sensitized skin had obvious erythema exudation，local flushing，skin thickening and scab formation.Compared with the model group，the traditional Chinese medicine group and the hormone group improved significantly.Pathological examination showed that the spinous layer of the model group was significantly thicker than that of the normal group，keratinized beads were increased，hair follicle structure was significantly increased，keratinized layer was significantly thicker，inflammatory cell infiltration and epidermal irregularity were observed.Keratinized layer and spinous layer were thicker in the hormone group and the traditional Chinese medicine group，and keratinized beads and hair follicle structure were slightly increased，which was significantly improved compared with the model group.The expression of PAR-2 and TRPA1 in skin tissue of the model group was significantly higher than that of the normal group(P<0.05).After intervention with Chinese medicines and hormones，the expression of PAR-2 and TRPA1 was significantly decreased(P<0.01).Compared with the normal group，PAR-2 and TRPA1 immunoreactivity in the skin tissue of the model group increased significantly，especially in keratinocytes.The expression of PAR-2 and TRPA1 in the traditional Chinese medicine group and the model group was lower than that in the model group.Conclusion:Mahuang Lianqiao Chixiaodou Decoction can improve AD skin lesion，relieve skin itching，down-regulate the expression of PAR-2 and TRPA1，and then regulate non-histamine dependent nerve signal transduction.