To investigate the effect and mechanism of Bushen Huoxue Decoction by studying the effect of Bushen Huoxue Decoction on ITP.Methods:the immune ITP model was established.The contents of immunoglobulin in intestinal mucus and the changes of P53 protein expression in mouse mesenteric lymph node lymphocytes were detected by ELISA.The changes of lymphocyte cycle and apoptosis rate in mesenteric lymph node were detected by flow cytometry.Results:Bushen Huoxue Decoction can significantly reduce the content of immunoglobulin in intestinal mucus of mice (P<0.05).The expression of P53 protein decreased,which was significantly different from the model group (P<0.01).The proportion of lymphocytes in G0/G1 stage significantly increased (P<0.05),the proportion of lymphocytes in S stage decreased (P<0.05),and the apoptosis rate of lymphocytes reduced (P<0.05),which indicated Bushen Huo xue Decoction was effective on the distribution of lymphocyte cycle and apoptosis.Conclusion:Bushen Huoxue Decoction has obvious therapeutic effect on ITP mice,the main mechanism of which is manifested on the down-regulation on the IgG and SIgA content of ITP mice and the expression of lymphocyte P53 protein,improvement of the distribution of lymphocyte cycle in ITP mice and reduction the apoptosis rate of lymphocyte,etc.The specific mechanism needs to be further explored.