To establish a high-performance liquid chromatography (HPLC) method for identification and content determination of Panax quinquefolium，Panax ginseng，and Panax notoginseng，which belongs to Araliaceae，to provide scientific basis for the identification and quality evaluation of the same medicinal materials.Methods:Panax quinquefolium，Panax ginseng，and Panax notoginseng were all extracted with methanol，and the feature map and content were under the same liquid chromatography conditions:C8 chromatographic column，acetonitrile-water as mobile phase，gradient elution，ultraviolet light (203 nm) detection，Chempattern chemometrics software was used for data processing，similarity calculation and principal component analysis (PCA).Results:The feature map of Panax quinquefolium had 11 common peaks，the similarity was 0.86-0.99，and the diagnostic component was ginsenoside Rc.The feature map of Panax ginseng had 9 common peaks，the similarity was 0.98-0.99，and the diagnostic component is ginsenoside Rf.The feature map of Panax notoginseng had 8 common peaks，the similarity was 0.96-1.0，and the diagnostic component is Panax notoginseng saponin R1.The similarity within the varieties was very high，and the similarity between the varieties was as low as 0.54.Principal component analysis could distinguish the 3 medicinal materials significantly，and display the diagnostic components and common components.The ginsenoside Rg1，Re，Rb1 and Rd peaks had a cumulative contribution weight value of 87% for distinguishing the 3 medicinal materials.Among them，the peak area ratios of ginsenoside Rg1 and ginsenoside Re were significantly different among the 3 medicinal materials，and the ratio range was stable.The ratios of the three were 0.13±0.08，1.20±0.60，and 8.30±3.0.Identification was significant.Regarding the monomer content and total content of each common component，Panax notoginseng was the highest，Panax quinquefolium was the second，and Panax ginseng is the lowest.The content of ginsenoside Rg1 in Panax quinquefolium was basically the same.Conclusion:The newly established HPLC feature map method determines the content of the main saponin components while identifying the same medicinal materials.The method is exclusive，has high stability，and has good repeatability.It also greatly shortens the analysis time and improves the analysis efficiency.Quality evaluation provides scientific and convenient methods and basis.