To observe whether ginsenoside Rb1 can inhibit senescence of lens epithelial cells by regulating expression of SIRT1.Methods:Senescence model of lens epithelial cell line SRA01/04 was established,and cultured with adding ginsenoside Rb1.The mRNA expression of SIRT1 was detected by PCR and the degree of senescence were detected by β-galactosidase staining positive rate.The expression of SIRT1 in the normal lens epithelial cell line SRA01/04 was knocked down to detect aging,and then ginsenoside Rb1 was added again to observe whether the above indicators have changed by PCR and immunofluorescence.Results:60 μmol/L ginsenoside Rb1 treatment of senescent SRA01/04 cells increased the expression of SIRT1 (P<0.05); after silencing SIRT1,SRA01/04 cells senescence significantly increased (P<0.05); compared with the silence SIRT1 group,after silencing SIRT1 simultaneously adding ginsenoside Rb1,SIRT1 increased.Conclusion:Ginsenoside Rb1 can resist the senescence of lens epithelial cells by regulating the expression of SIRT1.