To explore the efficacy and optimal effective dose range of Fufang Yizhihao Granules and provide the reference for the effectiveness and dosage of children's clinical medication.Methods:The anti-viral effect was observed through the pneumonia model and death model of young mice caused by H1N1 influenza virus infection; the anti-inflammatory effect was observed by the abdominal capillary permeability test and the cotton ball granuloma test; writhing test was used to observe its analgesic effect; Staphylococcus aureus infection-induced death model of young mice was used to observe its antibacterial effect,and LPS-induced young rabbit fever model was used to observe its antipyretic effect.Results:0.7 and 0.9 g/(kg·d)(content of crude drugs ) of Fufang Yizhihao Granules had stable antiviral and antibacterial effects,inhibited capillary permeability and formation of rat cotton granuloma,and reduced the number of writhing times in mice.Conclusion:Fufang Yizhihao Granules have significant antiviral,antibacterial,anti-inflammatory and analgesic effects on young animal models in the range of 0.7~0.9 g/(kg·d).