To explore the effects of Tongfengting Decoction on serum uric acid level, serum XO concentration, UAT mRNA and URAT1 mRNA expression of renal tubular epithelial cells of hyperuricemia rats, and study the specific mechanism of uric acid-lowering effect of Miao medicine Tongfengting Decoction. Methods:A total of 56 SD rats were randomly divided into a Tongfengting low dose group, a medium dose group and a high dose group, a blank group, a model group, an allopurinol group and a benzbromarone group. Except for the blank group, which was fed to the common diet daily, the other groups were prepared into hyperuricemia model. The model group and the blank group were given the same amount of normal saline, and the other groups were given the corresponding drugs. All rats were dosed for 7 days, and on the seventh day after dosing, the serum uric acid level, serum XO concentration, UAT mRNA and URAT1 mRNA expression of renal tubular epithelial cells of every group rats were detected. Results:Compared with the model group：1)on decreasing the XO：except for the benzbromarone group and the Miao medicine Tongfengting low dose group, and the other groups can reduce the serum XO concentration(P<001); the efficacy of the allopurinol group was similar to that of the Miao medicine Tongfengting high dose group and medium dose group(P>005). The efficacy of the high dose group and medium dose group of Tongfengting were better than that of the low dose group(P<005). 2)on reducing the uric acid level：except for the Miao medicine Tongfengting low dose group, the other treatment groups had better uric acid-lowering effect(P<001); the efficacy of reducing uric acid level of the Miao medicine Tongfengting high dose group, medium dose group and the allopurinol group, the benzbromarone group were similar(P>005). 3)on regulating the expression of UATmRNA：the UATmRNA expression was up-regulated in the Miao medicine Tongfengtin high dose group(P<001), and the other groups were not statistically significant(P>005). 4)on regulating the expression of URAT1mRNA：the URT1mRNA expressions were down-regulated in the Miao medicine Tongfengtin high dose group, medium dose group and the benzobromoma group(P<005), and the efficacy of these groups were similar(P>005). The other groups were not statistically significant(P>005). Conclusion:The Miao medicine Tongfengtin Decoction can reduce the serum uric acid concentration in hyperuricemia rats by inhibiting the activity of xanthine oxidase, up-regulating the expression of UATmRNA and down-regulating the expression of URAT1mRNA.