To screening potential active constituents against SARS-CoV-2 from Lonicera japonica Flos(LJF) by molecular docking technology and provide reference for discovery of lead compounds against SARS-CoV-2 and interpretation of its active substances for the treatment of COVID-19.Methods:The database of 382 chemical constituents from LJF was established based on the literature summary，combining TCMSP database retrieval.SARS-CoV-2 Mpro and SARS-CoV-2/angiotensin-converting enzyme 2(ACE2) were employed as molecular docking targets.Discovery Studio software was used for virtual calculation and the potential active components against SARS-CoV-2 were picked out on the basis of the ranking of docking score.Results:Triterpenoid saponins in LJF had strong affinity with SARS-CoV-2 Mpro and 7 compounds were potential inhibitors，taking SARS-CoV-2 Mpro crystal structure complex inhibitor N3 as the positive control.Iridoids and ceramides had a good blocking effect on SARS-CoV-S/ACE2 complex and 10 compounds were potential blockers.Conclusion:A total of 16 Potential active compounds against SARS-CoV-2 were found from LJF.The present study provides reference for clarifying effective substances of LJF against COVID-19，and discovering lead compounds against SARS-CoV-2 from LJF.