To use GEO chip data and traditional Chinese medicine inheritance auxiliary system to obtain the differential genes and traditional Chinese medicine prevention and treatment prescriptions of cerebral infarction of qi deficiency and blood stasis type，and to analyze its mechanism，so as to provide theoretical basis for the prevention and treatment strategy of traditional Chinese medicine of qi deficiency and blood stasis type of cerebral infarction.Methods:The prevention and treatment strategy of traditional Chinese medicine for cerebral infarction of qi deficiency and blood stasis was discussed by means of bioinformatics，data mining，network pharmacology and other interdisciplinary methods.Integrate GEO，TCMIP，GeneCards，PubChem，TCMSP，String，WebGestalt，DAVID multi-database resources and Cytoscape，R language software tools to screen disease targets and traditional Chinese medicine targets of qi deficiency and blood stasis type of cerebral infarction.From the point of view of PPI network，GO and KEGG enrichment，the mechanism of the basic side of qi deficiency and blood stasis type of cerebral infarction obtained by data mining of TCM inheritance auxiliary system were discussed.Results:A total of 2542 disease targets of cerebral infarction with qi deficiency and blood stasis were obtained.The results of differential genes showed that the expressions of HIF1A，PIK3CA，PIK3R1，TP53，NFKB1，HSP90AA1，CREBBP，CASP8，IL6，TNF and ITGB were up-regulated，while the expressions of APOE，IGF1，CXCL5，SRC，ADCY5 and PTGS2 were down-regulated，which can be used as potential targets for disease diagnosis and treatment.A total of 58 the medication rule of the first qi deficiency and blood stasis type of cerebral infarction prescription，including frequency，correlation and new prescription combination，to obtain the basic prescription of qi deficiency and blood stasis type of cerebral infarction，which is composed of Astragali Radix，Ligusticum chuanxiong Hort，Angelica sinensis (Oliv.) Diels，Pheretima，Codonopsis Radix，Panacis Quinquefolii Radix，Atractylodes macrocephala Koidz，Persicae Semen，Paeoniae Rubra Radix，Aurantii Immaturus Fructus，which have the effect of replenishing qi and activating blood circulation，promoting qi and resolving phlegm，and its action mechanism may be related to the regulation of differential genes in a multi-component，multi-functional and multi-channel way.It plays a role mainly through HIF-1，TNF，NF-kappaB，Apoptosis，PI3K-AKT and other pathways.Conclusion:The prevention and treatment of cerebral infarction of qi deficiency and blood stasis type by traditional Chinese medicine should focus on the target of different genes，select the clinical basic prescription，and add or subtract traditional Chinese medicine according to clinical syndrome differentiation.At the same time，multi-disciplinary interdisciplinary method can be used to verify the clinical and experimental results of traditional Chinese medicine in the treatment of qi deficiency and blood stasis cerebral infarction from multi-component，multi-pathway and multi-target levels.This study provides theoretical guidance for the treatment of qi deficiency and blood stasis cerebral infarction with traditional Chinese medicine.It is of great significance to promote the modernization of traditional Chinese medicine.