To establish quality standards and evaluate the anti-migraine effect of Xiongfu dripping pills.Methods:A HPLC method was used for simultaneous determination of 8 main components in Xiongfu dripping pills.The effects of Xiongfu dripping pills on the contents of 5-HT，β-EP，CGRP，ET-1，MMP-9，NF-κB，NOS and the mRNA expression of Calca and c-fos in migraine rats were evaluated.Results:The HPLC-DAD method determined the content of ferulic acid，senkyunolide I，senkyunolide A，3-butylphthalide，Z-ligustilide，Z-butylidenephthalide，cyperotundone and α-cyperone.The precision，repeatability and stability were favorable.The average recoveries and corresponding RSD values were 100.68（2.76%）、98.71（2.88%）、101.66（2.17%）、100.15（2.60%）、99.50（2.88%）、102.47（2.22%）、100.11（2.91%）、100.66（3.03%），respectively.Compared to normal rats，the levels of 5-HT and β-EP in migraine rats decreased significantly(P<0.01，P<0.01)，CGRP，ET-1，MMP-9，NF-κB and NOS increased significantly(P<0.05，P<0.05，P<0.01，P<0.01，P<0.01)，and the mRNA expression of Calca and c-fos also increased significantly(P<0.05，P<0.01).Xiongfu dripping pills significantly improved 5-HT and β-EP，reduced CGRP，ET-1，MMP-9，NF-κB，NOS and reduced the mRNA expression of Calca and c-fos to treat migraine.Conclusion:The HPLC method is simple，fast，accurate，and it can be used for the quality control of Xiongfu dripping pills.Xiongfu dripping pills treat migraine by regulating various neurotransmitters and vasoactive substances.