To explore the protective effects of berberine on the injury of gastric mucosal epithelial cells in mice model with helicobacter pylori.Methods:25 male BALB/c mice were selected and randomly divided into the control group，model group，low-dose experimental group，medium-dose experimental group and high-dose experimental group，with 5 in each group.The mice in the control group were given 0.2 mL/each with normal saline and brucella broth.The other four groups were all given 0.2 mL/each helicobacter pylori NCTC11637 bacterial solution to construct the helicobacter pylori infection model.After successful modeling，the low-dose experimental group，the middle-dose experimental group，and the high-dose experimental group were given 25 mg/kg，50 mg/kg and 100 mg/kg berberine，respectively.1 time per day for 2 consecutive weeks.The control group and model group were given sodium carboxymethyl cellulose solution at the dose of 5 mL/kg by gavage respectively.Mouse gastric mucosal epithelial cells viability was assessed using CCK-8.The levels of IL-1β，IL-8，IL-4，IL-10 in cell supernatant were detected by enzyme-linked immunoassay(ELISA).Cell apoptosis were analyzed by flow cytometry.The expression of Bax and Cl-caspase-3 in the cells was detected by western blotting.Results:Compared with the control group，cell viability decreased significantly in the model group(P<0.05)，while the contents of IL-1β，IL-8，IL-4，IL-10，apoptosis rate and expression of Bax and Cl-caspase-3 were significantly increased(P<0.05).Compared with the model group，cell viability was significantly increased in low，medium and high dose groups(P<0.05)，while the contents of IL-1β，IL-8，IL-4，IL-10，apoptosis rate and expression of Bax and Cl-caspase-3 were significantly decreased(P<0.05).Conclusion:Berberine can reduce the inflammatory damage of helicobacter pylori infection on gastric mucosal epithelial cells in mice.